TEV-Protease (recombinant)

InVivo offers a recombinant form of the TEV-Protease from Tobacco Etch Virus produced in
Escherichia coli. TEV-Protease is a highly specific cysteine protease with specificity for the
sequence ENLYFQ↓S (cleavage position indicated by a down arrow); however, the amino acid
in the P1’ position can also be G, A, M, C, or H.

The expression construct contains residues 2038 to 2279 of the TEV Genome polyprotein
(UniProt entry P04517), a natural cleavage product, called Nuclear inclusion protein A (Nia),
with a length of 272 amino acids. The protein includes a C-terminal V5-tag (GKPIPNPLLGLDST)
and hexa-histidine-tag.

The recombinant protein is produced with an E. coli expression system and purified using
affinity chromatography and size exclusion chromatography.

Recommended use:
Use 200 μL or 1 mg of TEV-Protease to cleavage 100 mg of substrate and incubate overnight. TEV-Protease
is maximally active at 34 °C, but reactions can be performed at room temperature or 2–8 °C with
acceptable loss of activity. TEV-Protease operates at pH values between 4–9 (ideally pH 6.0–8.5). A standard
reaction buffer is 50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, 1 mM DTT. TEV protease is sensitive to high salt
concentrations and loses high value of activity in the presence of 500 mM sodium chloride.

The product is for research use or for further manufacturing only.