Taq-Polymerase RecAb (TP7)

ID: RP_SZ_940 Category:

Taq-Polymerase RecAb (TP7) is a recombinant mouse antibody (mIgG2a,κ) that binds to Taq polymerase and inhibits the polymerase activity at room temperature.

The recombinant antibody is produced under serum‐free (animal component-free) conditions in CHO cells system and purified through one-step purification with Protein-A affinity chromatography.

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Taq-Polymerase RecAb (TP7) is a recombinant mouse antibody (mIgG2a,κ) that binds to Taq
polymerase and inhibits the polymerase activity at room temperature. The antibody is
inactivated and the enzyme released upon temperature increase (“hot start antibody”) during
normal PCR cycling conditions, allowing reactions to be set up at room temperature. The target
Taq polymerase (Uniprot: P19821) is produced by the thermophilic eubacterium Thermus
aquaticus and is a popular thermostable DNA polymerase used for PCR. The original
TP7 antibody was prepared by Scalice et al. (1994) through mouse immunization with
recombinant Taq expressed in E. coli.

The recombinant antibody is produced under serum‐free (animal component-free) conditions
in CHO cells system and purified through one-step purification with Protein-A affinity
chromatography.

We recommended to use TP7 antibody at a ≥ 1:1 molar ratio over Taq polymerase.

 

Product-ID: RP_SZ_940
Immunogen: recombinant TaqPol expressed in E. coli
Expression System: Mammalian; CHO
Isotype: Mouse IgG2a, kappa
Formulation: Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
Concentration: ≥ 0.5 mg/ mL
Purity: ≥ 90% (CGE, reducing conditions)
≤ 10 % aggregates (analytical SEC)
Storage: 2 - 8 °C

 

The product is for research use or for further manufacturing only.

Additional information

Literature

[1] A. S. Kaledin, A. G. Sliusarenko, and S. I. Gorodetskiĭ, “[Isolation and properties of DNA polymerase from
extreme thermophylic bacteria Thermus aquaticus YT-1].,” Biokhimiia, vol. 45, no. 4, pp. 644–51, Apr. 1980,
[Online]. Available: http://www.ncbi.nlm.nih.gov/pubmed/7378495.
[2] R. K. Saiki et al., “Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase,”
Science (80-. )., vol. 239, no. 4839, pp. 487–491, Jan. 1988, doi: 10.1126/science.2448875.
[3] K. Terpe, “Overview of thermostable DNA polymerases for classical PCR applications: From molecular and
biochemical fundamentals to commercial systems,” Appl. Microbiol. Biotechnol., vol. 97, no. 24, pp. 10243–
10254, 2013, doi: 10.1007/s00253-013-5290-2.
[4] E. R. Scalice, D. J. Sharkey, and J. L. Daiss, “Monoclonal antibodies prepared against the DNA polymerase from
Thermus aquaticus are potent inhibitors of enzyme activity,” J. Immunol. Methods, vol. 172, no. 2, pp. 147–163,
1994, doi: 10.1016/0022-1759(94)90102-3.
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Product Information Sheet – RP_SZ_940
Notice

The product is for research use or for further manufacturing only.

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