S1-RBD

ID: S1-RBD_HEK Category:

InVivo offers a recombinant form of the spike glycoprotein receptor binding domain (RBD) from severe acute respiratory syndrome-related coronavirus (SARS-CoV-2), Wuhan-Hu-1-isolate (MN908947), which is produced under serum-free conditions in HEK-INV cells (InVivo proprietary optimized; human embryonic kidney, HEK293 cells).

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InVivo offers a recombinant form of the spike glycoprotein receptor binding domain (RBD) from severe acute respiratory syndrome-related coronavirus (SARS-CoV-2), Wuhan-Hu-1-isolate (MN908947), which is produced under serum-free conditions in HEK-INV cells (InVivo proprietary optimized; human embryonic kidney, HEK293 cells).

The construct contains 223 residues of the SARS-CoV-2 spike glycoprotein RBD, representing amino acid residues 319 to 541 of before mentioned annotation. The protein includes a Cterminal hexa-histidine tag and is purified using immobilized metal exchange chromatography (IMAC) and preparative SEC (for polishing).

 

Product-ID: S1-RBD_HEK
Expression System: Mammalian; HEK
Protein Acession Number: UniProt: QHD43416.1
Amino Acids: 319-541
Mature Protein N-Term: Gln18 (predicted)
Tag: 6 x His-Tag; C-terminal
Expected Molecular Weight: 26 kDa (glycosylated form runs at 40-55 kDa in gel electrophoresis)
Formulation: Liquid ; 20 mM NaPP, 300 mM NaCl pH 7.2
Concentration: > 1 mg/ml
Purity: > 90% (via analytical CGE under reducing conditions)
Aggregation Level: < 10% (via analytical SEC)

 

The product is for research use or for further manufacturing only.

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Product Information Sheet – S1-RBD_HEK

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The product is for research use or for further manufacturing only.

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