Human Thrombomodulin, extracellular domain, sequence modified (recombinant)

ID: PR_SZ_160 Category:

InVivo offers a recombinant form of the extracellular domain of Human Thrombomodulin.

The recombinant protein is produced under serum‐free conditions in optimized HEK293-cell system and purified through one-step purification with ion exchange chromatography.

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InVivo offers a recombinant form of the extracellular domain of Human Thrombomodulin
which is produced under serum-free conditions in HEK 293 (human embryonic kidney) cells.
The expression construct contains residues 1 to 515 of UniProt entry P07204: M406L, S492A.

The M406L substitution (M388L in the mature protein) leads to a Thrombomodulin variant
that resists oxidative inactivation. The S492A substitution (Ser474 in the mature protein)
removes an O-glycosylation site for chondroitin sulfate, a glycosaminoglycan (GAG) that
increases anticoagulant activity.

The recombinant protein is produced under serum‐free conditions in optimized HEK293-cell
system and purified through one-step purification with ion exchange chromatography.

 

Product-ID: RP_SZ_160
Expression System: Mammalian; HEK
Protein Accession # : P07204
Amino Acids: Met1–Ser515 (Extracellular Domain of Thrombomodulin)
Mutations: M406L, S492A
Mature Protein N-Term: Ala19 (predicted)
Tag: none
Expected Molecular Weight: 52 kDa (runs at 60-85 kDa on SDP-PAGE under reducing conditions)
Formulation: Liquid, 25 mM sodiumacetate, 150 mM sodiumchloride,
0.01 % Tween® 80, pH 6.0
Concentration: ≥ 1.0 mg/ mL
Purity: -/-

 

The product is for research use or for further manufacturing only.

Additional information

Literature

[1] C. B. Glaser et al., “Oxidation of a specific methionine in thrombomodulin by activated neutrophil products
blocks cofactor activity. A potential rapid mechanism for modulation of coagulation” J Clin Invest, vol. 90, no.
6, pp. 2565-73, 1992. PMID: 1334978
[2] J. F. Parkinson et al., “Recombinant human thrombomodulin. Regulation of cofactor activity and
anticoagulant function by a glycosaminoglycan side chain” Biochem J, vol. 283, no. 1, pp. 151-7, 1992. PMID:
1314561
[3] T. Edano et al., “Increased anticoagulant activity of recombinant thrombomodulin modified with
glycosaminoglycan” Biol Pharm Bull., vol. 21, no. 4, pp. 375-81, 1998. PMID: 9586576
Document

Product Information Sheet – RP_SZ_160
Notice

The product is for research use or for further manufacturing only.

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