HLA-A,B,C mAb (W6/32), InVivoPure

Endotoxin level ≤ 2 EU/mg

The HLA-A,B,C mAb (W6/32) monoclonal antibody reacts with the human major
histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta
2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated
immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic
epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43
kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus
monkey.
MHC class I plays a crucial role in the adaptive immune response by presenting endogenous
antigens to cytotoxic CD8 T cells. The T cell receptor (TCR)/CD3 complex of CD8 T cells
interacts with peptide MHC class I, which induces CD8 T cell activation and subsequent cell
killing. CD8 molecules also bind to MHC class I, which enhances TCR signaling. In contrast to
CD8 T cells, MHC class I is an inhibitory ligand for natural killer cells (NK cells) and promotes selftolerance.

This antibody is produced exclusively under serum-free conditions from hybridoma and
purified with Protein-A or Protein-G affinity chromatography.
Applications Reported: This W6/32 antibody has been reported for use in flow cytometric
analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections.

The product is for research use only and not for use in diagnostic or therapeutic procedures.

The HLA-A,B,C monoclonal antibody (clone W6/32) was purified from supernatant of HB- 95™ W6/32 from the ATCC. The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.