Endotoxin level ≤ 2 EU/mg
The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface
receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic
cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important
role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4
associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is
blocked using the RM4-5 antibody by competing with GK1.5 for the same binding site on the
CD4 molecule. GK1.5 significantly enhanced the percentages of peripheral CD4+CD25+Foxp3+
Treg cells in CD4+ T cells in mice without altering their immunosuppressive function.
The antibody profoundly blocks antigen specific murine class II MHC antigen reactive helper T
lymphocyte lines.
This antibody is produced exclusively under serum-free conditions from hybridoma and
purified with Protein-A or Protein-G affinity chromatography.
Product-ID: |
AK3602P |
Clone: |
GK1.5 |
Immunogen: |
Animals were immunized with the cloned cytotoxic T lymphocyte lines V4 and 243/2.5. L3T4 is expressed on mouse helper/inducer T cells and is analogous to the human Leu-3/T4 molecule. |
Host: |
Rat |
Clonality: |
Monoclonal |
Isotype: |
IgG2b κ |
Formulation: |
Clear Liquid, PBS, pH 7.4, 0.2 μm sterile filtered |
Concentration: |
≥ 1.00 mg/mL |
Purity: |
≥ 90 % (CGE, reducing conditions) |
|
≤ 10 % aggregates (analytical SEC) |
Endotoxin: |
≤ 2 EU/mg (LAL test) |
Storage: |
2 - 8 °C |
The product is for research use only and not for use in diagnostic or therapeutic procedures.
The CD4 monoclonal antibody (clone GK 1.5) was purified from supernatant of TIB-207™ GK1.5 from the ATCC. The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.
Reviews
There are no reviews yet.