CD16/CD32 mAb (2.4G2), InVivoPure

Endotoxin level ≤ 2 EU/mg

CD16/CD32 (2.4G2) rat antibody recognizes endogenous levels of total CD16/CD32 protein. This
antibody detects an epitope within the extracellular domain and is expected to detect all isoforms
of CD16 and CD32. The 2.4G2 antibody blocks the interaction between IgG and CD16 or CD32.
Therefore pre-treatment with the 2.4G2 antibody is commonly described in protocols for
minimizing non-specific binding of antibodies to Fc receptor-bearing cells. This is particularly useful
in applications such as flow cytometry, cell sorting, and immunofluorescence. CD16/CD32 is
expressed on NK cells, monocytes, macrophages, dendritic cells (at low levels), kupffer cells,
granulocytes, mast cells, B lymphocytes and some activated T lymphocyte.

This antibody is produced exclusively under serum-free conditions from hybridoma and purified
with Protein-A or Protein-G affinity chromatography.

The product is for research use only and not for use in diagnostic or therapeutic procedures.

The CD16/CD32 monoclonal antibody (clone 2.4G2) was purified from supernatant of HB-197™ 2.4G2 from the ATCC. The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.