Human IgE (recombinant)

ID: RP_SZ_361 Category:

Recombinant human IgE antibody (hIgEλ), whose amino acid sequences are identical to the first-described IgE antibody from a myeloma patient with initials N.D.

The recombinant antibody is produced transiently under serum‐free conditions in optimized HEK293-cell system and purified through one-step purification with affinity chromatography.

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Recombinant human IgE antibody (hIgEλ), whose amino acid sequences are identical to the
first-described IgE antibody from a myeloma patient with initials N.D, who was hospitalized in
1965. Thus, the antibody is also referred to as IgND or IgE (ND). From the blood of
patient N.D., a derived hIgEλ-producing myeloma cell line 266 BL or U-266 was developed.
The corresponding heavy and light chain sequences were determined and are published under
Genbank entries AH005278.2 and X51754, respectively.

The recombinant antibody is produced transiently under serum‐free conditions in optimized
HEK293-cell system and purified through one-step purification with affinity chromatography.

 

Product-ID: RP_SZ_361
Expression System: Mammalian; HEK
Isotype: Human IgE
Subclass: hIgEλ2
Protein Accession #: HC: AH005278.2 translation (residues 19–574)
LC: X51754 translation (residues 20–235)
Formulation: Liquid, PBS, pH 7.4, 0.2 μm sterile filtered
Concentration: ≥ 0.5 mg/ mL
Purity: ≥ 90 % (CGE, reducing conditions)
< 5 % aggregates (Analytical SEC)

 

The product is for research use or for further manufacturing only.

Additional information

Literature

[1] S. G. Johansson and H. Bennich, “Immunological studies of an atypical (myeloma) immunoglobulin.,”
Immunology, vol. 13, no. 4, pp. 381–94, 1967. PMID: 4168094.
[2] S. G. Johansson, H. Bennich, and L. Wide, “A new class of immunoglobulin in human serum.” Immunology.
1968;14: 265–72. PMID: 4170889.
[3] H. Bennich and S. G. O. Johansson, “Structure and Function of Human Immunoglobulin E,” in Symposium on
Fundamental Cancer Research, vol. 37, 1971, pp. 1–55. PMID: 5004326.
[4] K. Nilsson, H. Bennich, S. G. Johansson, and J. Pontén, “Established immunoglobulin producing myeloma (IgE)
and lymphoblastoid (IgG) cell lines from an IgE myeloma patient.,” Clin. Exp. Immunol., vol. 7, no. 4, pp. 477–
89, 1970. PMID: 4097745.
[5] J. H. Kenten et al., “Cloning and sequence determination of the gene for the human immunoglobulin ε chain
expressed in a myeloma cell line,” Proc. Natl. Acad. Sci. U. S. A., vol. 79, no. 21 I, pp. 6661–6665, 1982. PMID:
6815656
[6] M. Seno et al., “Molecular cloning and nucleotide sequencing of human immunoglobulin epsilon chain
cDNA.,” Nucleic Acids Res., vol. 11, no. 3, pp. 719–26, Feb. 1983. PMID: 6300763
[7] T. J. Vasicek and P. Leder, “Structure and expression of the human immunoglobulin lambda genes.,” J. Exp.
Med., vol. 172, no. 2, pp. 609–620, Aug. 1990. PMID: 2115572.
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Product Information Sheet – RP_SZ_361
Notice

The product is for research use or for further manufacturing only.

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