Anti-PCT clone P298-3-1-73-09

Anti-PCT clone P298-3-1-73-09 is a mouse monoclonal antibody against human Procalcitonin
(PCT), a 116-amino acid peptide precursor of the hormone calcitonin, that is used as a
biomarker to aid in diagnosis of bacterial infection or sepsis. PCT is produced after cleavage
of the 25-amino acid signal peptide from pre-procalcitonin (UniProt entry: P01258), the gene
product of the CALC-1 (CALCA) gene. PCT consists of three sections; the amino terminus (57
amino acids), immature calcitonin (33 amino acids) and katacalcin (21 amino acids).

In healthy individuals, transcription and translation of the CALC-1 gene is mostly confined to the
thyroid C-cells. However, in response to bacterial infection, mediated by cytokines interleukin-
6 (IL-6), tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-β), PCT production is activated
in all parenchymal tissues. These tissues lack the ability to cleave PCT to its mature form,
calcitonin, leading to accumulation of PCT. In addition, PCT production is attenuated by
interferon-γ primarily secreted in response to viral infection. This characteristic makes PCT a
specific marker for bacterial infection.

The antibody is produced exclusively under serum-free conditions from hybridoma and purified
through one-step purification with Protein-A affinity chromatography.

The product is for research use or for further manufacturing only.